HANDS-ON WORKSHOP ON REAL-TIME qPCR

Event Date : 24/02/2023 - 25/02/2023
Event Time : 8:00 am - 5:00 pm
Materials : Download PDF
  • 24 February 2023 (Friday)
    2:45pm – 5:30pm (GMT +8)
    Online lecture sessions via Zoom
  • 25 February 2023 (Saturday)
    8:30am – 3:00pm
    Onsite hands-on at International Medical University, Bukit Jalil, Kuala Lumpur, Malaysia

In Collaboration With:

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Two parts make up this unified workshop is aimed to deliver

  • Foundational lectures covering aspects of good sample preparation, distinct detection systems (SYBR vs. PROBE), and a wide range of real-time qPCR applications.
  • ¬†Nucleic acid extraction techniques using cutting-edge magnetic bead technology and SYBR real-time qPCR for semiquantitative data analysis in the hands-on lab session. Participants will benefit from the grounded experiential learning provided by the combination of learning theory along with achieving hands-on experience that will better able them to implement these strategies in their own research practises.

Objectives

By the end of this workshop, participants will be able to:

  • Explain the working principle, standard operating procedure, troubleshooting techniques and applications of RT-qPCR.
  • Extract total DNA from human buccal swab samples.
  • Quantify and standardize the DNA sample.
  • Familiarize with preparation of reagent and master mix.
  • Interpret real time qPCR results and perform analysis.

Speakers

Casimir Pang Jun Rui
Field Application Specialist

Dr Tan Ju Lin
Field Application Specialist

REGISTRATION FEE (inclusive of SST)

Early Bird (Before 24 Jan 2023) RM650
Regular RM750
IMU Staff / Alumni / Student (Non-IMU) (Limited Seats) RM525
IMU Student (Limited Seats) RM375
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