• 24 February 2023 (Friday)
  2:45pm – 5:30pm (GMT +8)
  Online lecture sessions via Zoom
• 25 February 2023 (Saturday)
  8:30am – 3:00pm
  Onsite hands-on at International Medical University, Bukit Jalil, Kuala Lumpur, Malaysia

In Collaboration With:

Two parts make up this unified workshop is aimed to deliver

• Foundational lectures covering aspects of good sample preparation, distinct detection systems (SYBR vs. PROBE), and a wide range of real-time qPCR applications.
•  Nucleic acid extraction techniques using cutting-edge magnetic bead technology and SYBR real-time qPCR for semiquantitative data analysis in the hands-on lab session. Participants will benefit from the grounded experiential learning provided by the combination of learning theory along with achieving hands-on experience that will better able them to implement these strategies in their own research practises.

Objectives

By the end of this workshop, participants will be able to:

• Explain the working principle, standard operating procedure, troubleshooting techniques and applications of RT-qPCR.
• Extract total DNA from human buccal swab samples.
• Quantify and standardize the DNA sample.
• Familiarize with preparation of reagent and master mix.
• Interpret real time qPCR results and perform analysis.

Speakers

Casimir Pang Jun Rui
Field Application Specialist

Dr Tan Ju Lin
Field Application Specialist